Detailed view for TGME49_254370

Basic information

TDR Targets ID: 261940
Toxoplasma gondii, guanylyl cyclase
Source Database / ID:  ToxoDB 

pI: 6.6157 | Length (AA): 4367 | MW (Da): 477037 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 19

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00211   Adenylate and Guanylate cyclase catalytic domain
PF16209   Phospholipid-translocating ATPase N-terminal
PF16212   Phospholipid-translocating P-type ATPase C-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0035556   GO:intracellular signal transduction  

GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0016849   phosphorus-oxygen lyase activity  
GO:0005524   ATP binding  
GO:0003824   catalytic activity  
GO:0000166   nucleotide binding  
GO:0009190   cyclic nucleotide biosynthetic process  
GO:0008152   metabolic process  
GO:0007242   intracellular signaling cascade  
GO:0006754   ATP biosynthetic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 12 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1314 1505 4ke2 (A) 3 194 11.00 0 0.01 0.0920661 -0.09
1319 1561 2kr0 (A) 134 404 30.00 0.92 0.14 -0.0680554 1.5
1346 1427 2pne (A) 15 78 48.00 0.51 0.28 0.213077 -0.04
1796 2456 2zxe (A) 340 969 16.00 0 0.89 0.181462 0.94
2044 2229 4hqj (A) 575 739 28.00 0.83 1 0.162892 0.72
2197 2229 2rbk (A) 206 239 52.00 0.85 0.61 0.532857 0.18
2932 3194 3et6 (A) 467 650 42.00 0 1 0.0425244 0.4
3114 3194 1azs (B) 995 1075 43.00 0.0000000073 0.71 0.419848 0.22
3285 3498 5cwm (A) 5 207 24.00 0.71 0.97 0.303304 -0.35
3358 3510 5cwj (A) 2 161 14.00 0.71 0.16 0.258135 -1.3
4020 4210 2w01 (A) 439 624 25.00 0 0.99 0.386837 -0.83
4022 4187 4ni2 (A) 479 634 41.00 0 1 0.499312 -0.59

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_133188)

Species Accession Gene Product
Babesia bovis BBOV_I000770   adenylate and guanylate cyclase catalytic domain containing protein
Cryptosporidium hominis Chro.30141   guanylyl cyclase-related
Cryptosporidium parvum cgd3_1110   P-type ATpase fused to two adenyl cyclase domains and 21 predicted transmembrane regions
Neospora caninum NCLIV_008660   adenylate and guanylate cyclase catalytic domain- containing protein, putative
Plasmodium berghei PBANKA_0910300   guanylyl cyclase, putative
Plasmodium falciparum PF3D7_1138400   guanylyl cyclase
Plasmodium knowlesi PKNH_0936600   guanylyl cyclase, putative
Plasmodium vivax PVX_092535   Adenylate and Guanylate cyclase catalytic domain containing protein
Plasmodium yoelii PY04459   guanylyl cyclase-related
Plasmodium yoelii PY04450   guanylyl cyclase-related
Plasmodium yoelii PY04451   guanylyl cyclase-related
Toxoplasma gondii TGME49_254370   guanylyl cyclase
Theileria parva TP02_0848   guanylyl cyclase, putative

Essentiality

TGME49_254370 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0910300 Plasmodium berghei Slow plasmo
TGME49_254370 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_254370 (Toxoplasma gondii), guanylyl cyclase
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